Don’t stop at Native ChIP, complete the picture with Mass Spectrometry

We are very pleased to announce that we can now add mass spectrometry to our range of downstream applications. Our Chromatrap® Native ChIP kit is now fully compatible for mass spectrometry (MS) as a downstream process. Combining the solid state technology with MS enables researchers to obtain high quality datasets for chromatin proteomic profiling and mapping of histone modification patterns.

Technique Background

Chromatin immunoprecipitation (ChIP) is an immunoprecipitation technique commonly used for mapping the DNA-protein interactions in cells which are crucial for gene regulation.

The technique enriches DNA fragments to which a specific protein of interest or histone modification is bound. Enriched sites can then be identified by qPCR, hybridisation to a microarray (ChIP-on-chip), or by sequencing.

In our post-genomic era, mass spectrometry has become an invaluable versatile tool in the analysis of biomolecules. As a popular method of choice by researchers, MS is widely used to identify chromatin binding proteins and elucidate histone modification patterns.

In native ChIP (N-ChIP), the naturally occurring DNA-protein complexes are preserved by removing the need to fix cells prior to extraction. Although there are a number of published protocols outlining methods to performing N-ChIP, Chromatrap uniquely offers researchers an efficient kit with an optimized protocol specific for the technique. 

The Chromatrap® Technology

N-ChIP with Chromatrap efficiently ensures a high yield of DNA so samples can be analysed by multiple downstream processes. The unique solid-state technology eliminates the use of magnetic or agarose beads allowing users to quickly and easily recover ready-to-use, purified chromatin for downstream application including MS.

Coupling MS to ChIP will allow researchers to shed a light on the proteomic landscape of chromatin where we can begin to understand how protein interacts with repressed and active regions of the genome and their role in global gene regulation.  

Complete the picture with Native ChIP and Mass Spectrometry – perfect your N-ChIP technique with our Native ChIP kit and find out more about the range of assay kits we offer from our ChIP products page. If you have any questions, or want to find out more information about our solid state technology, you can also contact the Chromatrap team.