Chromatrap is a new, quicker, easier and more efficient way of performing ChIP assays. Chromatrap uses spin columns or 96 well filter plates and each column or well contains an inert porous polymer disc that is functionalised with Protein-A or Protein-G.
With pores designed to maximize the binding capacity of antibody-chromatin complexes, all Immunocapture can occur on the disc. Washing with three buffers and a simple elution step are all that is required to obtain the selectively enriched DNA, making Chromatrap® more efficient in the laboratory.
Unlike bead-based ChIP, Chromatrap eliminates common manual handling problems and the need for time-consuming blocking steps to reduce non-specific binding. In under 5 hours from chromatin loading to qPCR or sequencing, Chromatrap significantly reduces the assay time for ChIP, enabling more samples to be analysed increasing laboratory throughput and efficiency.
The Chromatrap patented format is unique and has been granted in the UK (Patent No. GB2482209), the US (Patent No. 9523681), China (Patent No. ZL 2011 8 0067254.X) and Japan (Patent No. JP 6088434) and Australia (Patent No. AU 2011340263).