Chromatrap® is delighted to announce a new patented protocol that is able to quickly and efficiently enrich transcription factors from native chromatin.
Native chromatin Immunoprecipitation (N-ChIP) is an alternative to the traditional cross-linked method (X-ChIP), which removes the need for fixing cells with formaldehyde prior to extraction. As a result the cells remain in a more natural 'native state'.
In honour of this new launch, Chromatrap’s® Product Development Manager, Amy Beynon, explains the benefits of the new Chromatrap® N-ChIP kit.
What does it do?
N-ChIP kits provide an alternative to traditional cross-linked ChIP, which removes the need for fixing cells prior to extraction. The Native ChIP kit can be used for the study of histone modifiers and certain abundant transcription factors that are likely to be bound to DNA that don’t require cross linking.
What makes the N-ChIP kit different?
For this kit we use a solid state platform to perform the ChIP assay and this can be in a spin column or 96 well filter plate. By using Chromatrap’s® Native ChIP kit this provides a quicker, easier and more efficient way of performing ChIP assays from Native Chromatin.
It also has unique patented technology which maximises the capture efficiency of the target chromatin/antibody complex. Unlike other approaches, this kit avoids the use of magnetic or agarose beads, so the non-specific background is eliminated, together with reduced manual handling errors and subsequent sample loss.
The wide dynamic range also allows for better results from small sample sizes and provides greater flexibility, thus more IP assays can be performed from just one sample. As a result the Chromatrap® N-ChIP kit sets a new standard for enriching transcription factors from native chromatin.
What are the benefits?
There are many benefits to using this kit. To start with, it is a lot quicker and easier to use, plus there are no manual handling steps.
In addition, the N-ChIP kit does not chemically fix your cells with formaldehyde, which means that the cells remain in a more natural and native state.
In some cases, there is increased affinity of antibody binding to antigens on native chromatin as it is more accessible.
Who should use this kit?
It is dependent on the research subject, but it is helpful to researchers that want to look at histone modifications.
For more information about the N-ChIP kit please download the protocol - if you have any specific questions contact our Technical Support team and they will be happy to help. You can also browse our full range of ChIP kits and order yours online today.