Native Chromatin Immunoprecipitation (N-ChIP) is an alternative to traditional cross-linked ChIP, which removes the need for fixing cells prior to extraction. The DNA/protein fragments are selectively immunoprecipitated using antibodies directed against the protein of interest and the resulting fractions treated to separate the DNA and protein components.
Highlights of Protocol
No need for fixation of cells
Able to ChIP transcription factors from native chromatin
Removes the need for agarose or magnetic beads resulting in more sample retained during the extraction process
Increased affinity of antibody binding to antigens on native chromatin as peptides are more accessible