Chromatrap Native ChIP protocol v1.2

Native Chromatin Immunoprecipitation (N-ChIP) is an alternative to traditional cross-linked ChIP, which removes the need for fixing cells prior to extraction. The DNA/protein fragments are selectively immunoprecipitated using antibodies directed against the protein of interest and the resulting fractions treated to separate the DNA and protein components. 

Highlights of Protocol

No need for fixation of cells

Able to ChIP transcription factors from native chromatin

Removes the need for agarose or magnetic beads resulting in more sample retained during the extraction process

Increased affinity of antibody binding to antigens on native chromatin as peptides are more accessible