Are you fixing your samples?
Are you doing qPCR and sequencing as a downstream process?
Are you using sonication or enzymatic digestion?
Sonication is a simple and effective method of chromatin shearing which provides randomly fragmented chromatin. Provided the temperature is controlled during the sonication process and emulsification is avoided good quality chromatin can be obtained from most cell types using this method.

Enzymatic shearing is useful if a sonicator is not available and is less disruptive to the epitopes of the protein of interest recognized by the specific antibody. Enzymatic shearing is essential when carrying out native ChIP (chromatin which has not been cross linked) as sonication can disrupts the protein/DNA complexes. Certain cell types may be resistant to lysis resulting in poor enzymatic shearing efficiency in this instance try sonication.
Are you using sonication or enzymatic digestion?
Sonication is a simple and effective method of chromatin shearing which provides randomly fragmented chromatin. Provided the temperature is controlled during the sonication process and emulsification is avoided good quality chromatin can be obtained from most cell types using this method.

Enzymatic shearing is useful if a sonicator is not available and is less disruptive to the epitopes of the protein of interest recognized by the specific antibody. Enzymatic shearing is essential when carrying out native ChIP (chromatin which has not been cross linked) as sonication can disrupts the protein/DNA complexes. Certain cell types may be resistant to lysis resulting in poor enzymatic shearing efficiency in this instance try sonication.
Would you like positive antibodies and primers in the kit?
Are you using FFPE tissue?
Are you using sonication or enzymatic digestion?
Sonication is a simple and effective method of chromatin shearing which provides randomly fragmented chromatin. Provided the temperature is controlled during the sonication process and emulsification is avoided good quality chromatin can be obtained from most cell types using this method.

Enzymatic shearing is useful if a sonicator is not available and is less disruptive to the epitopes of the protein of interest recognized by the specific antibody. Enzymatic shearing is essential when carrying out native ChIP (chromatin which has not been cross linked) as sonication can disrupts the protein/DNA complexes. Certain cell types may be resistant to lysis resulting in poor enzymatic shearing efficiency in this instance try sonication.
Would you like positive antibodies and primers in the kit?

Now that you know which of our kits is right for your research, take a look at our ChIP products and get started! If you have any further queries about our ChIP kits, then head over to our FAQ section or contact our tech support team directly.

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