The success of a ChIP assay is highly dependent on the quality of chromatin prepared. The sonication shearing kit provides buffers to optimise the shearing conditions of your chromatin by sonication.
Isolation of good quality, suitably fragmented chromatin is the most important prerequisite for a successful ChIP assay. Chromatin can be sheared enzymatically or by mechanical methods such as sonication. Shearing by sonication can be advantageous for difficult to lyse cell types, It also provides random sample fragmentation preventing any bias.
The Chromatrap® Sonication Shearing Kit provides an excellent method for the preparation of high quality, ideally fragmented chromatin for ChIP analysis.
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Kit Contents: 1.3M Glycine, Hypotonic Buffer, Lysis Buffer, 5M NaCl, 1M NaHCO3, Protease Inhibitor Cocktail (PIC), Proteinase K and Proteinase K Stop Solution.