The Chromatrap® Protein Extraction kit is a fast and efficient method of extracting cytoplasmic, nuclear or total protein from mammalian cells. The optimised reagents and protocol provide a high yield of protein with low cross-fraction contamination in under 1.5 hours.
Fast - Non-denatured, active proteins purified in 90 minutes.
Simple - No ultracentrifugation over gradients
Efficient - No ‘cross-fraction contamination’
Compatible – Perfect for Western blotting, gel-shift assays, protein assays, reporter gene assays and enzyme activity assays.
2 Reagents Supplied
Sufficient reagents are provided to perform 100 cytoplasmic and nuclear or total protein extractions. From 8 million cells, the kit typically yields 1000 µg cytoplasmic and nuclear protein and 2000 µg total protein