For complete disruption of cytoplasmic and nuclear membranes, Chromatrap®’s unique optimised buffer chemistry provides the solution. The detergent based buffers are ideal for effective fragmentation of nuclear material by sonication for a range of different species of organism and difficult to lyse cell types and tissues. Correct shearing is critical to the success of a ChIP assay.
The lysis and hypotonic buffers for sonication are compatible with all of Chromatrap® ChIP kits for sonication from fixed cells or tissue.