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ChIP-Seq

Chromatrap®’s new ChIP-Seq kit utilises the solid state technology to deliver a streamlined ChIP-Seq protocol from small cell numbers and low chromatin concentrations.

Specifically adapted for broader chromatin concentrations, Chromatrap® ChIP-Seq now combines the dynamic range of Chromatrap® with the downstream analysis power of deep sequencing. This allows faster, more reproducible genome wide identification of transcription factor binding sites and specific DNA associated protein modifications. The kit is ideal for more difficult primary cell samples and for the enrichment of low abundant transcription factors.

The ChIP-Seq kit is our more sensitive kit especially for low abundant transcription factors, and can be used for both qPCR and sequencing.  This kit is compatible with both high and low chromatin loadings with an increased slurry volume of 1ml offering greater flexibility with more difficult samples. This kit requires a clean-up step but due to the increased binding of the column sufficient DNA is obtained from one column without the need to pool samples for library preparation.

  • Compatible for both qPCR and sequencing as a downstream process
  • Has a wide dynamic range suitable for low and high chromatin loadings (1-50µg), which allows for better results from small sample sizes and providers greater flexibility
  • Allows user to perform up to 24 ChIP assays from cell collection through to immunoprecipitation, including up to 10 chromatin sample preparations.
  • Kit provides all the major components required for performing ChIP assays and DNA purification columns to obtain high quality DNA for NGS library preparation
  • Positive and negative antibody and primer sets are provided in the kit

Available in enzymatic format for shearing of DNA by enzymatic digestion and a 96 well microplate format for high throughput ChIP-SEQ.

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Not sure our ChIP-seq kit is the right product for you? Find out which Chromatrap® ChIP is best for your research.

Products
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Chromatrap ChIP-seq Protein A
Product Code : 500189
$616.00
Chromatrap ChIP-seq Protein A
Product Code : 500189
$616.00
Download Protocol
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Kit Contents: Chromatrap® Protein A spin columns (24), Column Conditioning Buffer, Wash Buffer 1, Wash Buffer 2, Wash Buffer 3, 1.3M Glycine, Lysis Buffer, ChIP-seq Elution Buffer, 0.1M NaOH, 5M NaCl, 1M NaHCO3, Hypotonic Buffer, Protease Inhibitor Cocktail (PIC), Proteinase K stop solution, Proteinase K, 1.5ml Collection tubes. DNA purification columns (24), DNA Binding Buffer, DNA Wash Buffer and DNA Elution Buffer. The kit also includes antibody validation controls: H3K4me3 and IgG along with a Forward and Reverse primer set for GAPDH (human promoter region).

Chromatrap ChIP-seq Protein G
Product Code : 500190
$616.00
Chromatrap ChIP-seq Protein G
Product Code : 500190
$616.00
Download Protocol
+ Add To Basket

Kit Contents: Chromatrap® Protein G spin columns (24), Column Conditioning Buffer, Wash Buffer 1, Wash Buffer 2, Wash Buffer 3, 1.3M Glycine, Lysis Buffer, ChIP-Seq Elution Buffer, 0.1M NaOH, 5M NaCl, 1M NaHCO3, Hypotonic buffer, Protease Inhibitor Cocktail (PIC), Proteinase K stop solution, Proteinase K, 1.5ml Collection Tubes. DNA purification columns (24), DNA Binding Buffer, DNA Wash Buffer and DNA Elution Buffer. The kit also includes antibody validation controls: H3K4me3 and IgG along with a Forward and Reverse primer set for GAPDH (human promoter region).

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