06 Jul 2014
Step by step guide to performing Chromatin Immunoprecipitation with Chromatrap

This live demonstration of the Chromatin Immunoprecipitation (ChIP) assay uses Chromatrap’s innovative solid-state ChIP kit. The video was prepared to inform researchers of an optimised protocol for preparing fresh chromatin from a dish of growing cells and how to verify the quality of the chromatin recovered. In addition each simple step in the Chromatrap procedure is shown, leading to measurable amounts of high-purity chromatin ready for PCR or sequencing.

The Chromatrap® kit does not use beads to separate the chromatin, it is much faster, easier to use and less prone to manual handling errors. This is demonstrated in the new video. The simple centrifugation and wash steps are seen being performed on the immunocaptured chromatin, which is held on the novel solid-state disc at the heart of each Chromatrap spin column.

This video is an essential primer for those contemplating the technique, but who have yet to perform a ChIP assay. In addition the video offers users of traditional bead-based methods the chance to see for themselves how much easier and quicker their ChIP assays can become in the lab by using Chromatrap spin columns or 96-well filter plates.

Browse our catalogue of revolutionary solid state platform ChIP kits and order now to make your ChIP assays just as quick and easy. Not sure which which Chromatrap® ChIP is best suited for you? Consult our step-by-step guide.