FFPE-ChIP utilises chromatin extracted from Formalin Fixed Paraffin Embedded (FFPE) tissue. Tissues, such as biopsies, are routinely fixed in high formaldehyde concentration and embedded in paraffin in hospitals under non standardised conditions to allow long term storage without excessive sample degradation. Therefore, a large amount of archived material is available to study. FFPE material has long been used for DNA and RNA analyses but due to difficulties in extracting proteins retaining their antigenicity, ChIP has not been possible until recently (Fanelli et al., 2011).
A shorter protocol
Increased chromatin yield and accessible epitopes
Greater flexibility in chromatin sample loading for ChIP
DNA purification reagents and columns now included for user convenience
To find out more, download the full PDF version of this protocol.