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Chromatrap® Enzymatic ChIP-Seq 96 well Microplate kit

Chromatrap® Enzymatic ChIP-Seq 96 combines the solid state technology in 96 well format with high throughput sequencing to deliver a streamlined ChIP-seq protocol from small cell numbers and low chromatin concentrations.

The unrivalled flexibility of experimental design offered by Chromatrap® ChIP-Seq 96 allows large reliable data sets to be collected efficiently enabling widespread effects to be studies across multiple sample and cell types all carried out simultaneously.

The Chromatrap® 96 Enzymatic ChIP-seq kit allows the user to perform up to 96 ChIP assays from cell collection through to immunoprecipitation, including up to 10 chromatin sample preparations by enzymatic digestion. The kit provides all of the major components required for performing ChIP assays including a DNA purification plate to obtain high quality DNA for NGS library preparation. Positive and negative antibody and primer sets are provided in the kit to give you satisfaction of chromatin preparation. With selective and sensitive enrichment of low chromatin loading and optimised elution buffer chemistry allows for high quality and quantity of immunoprecipitated DNA.

  • This kit is compatible for both qPCR and sequencing as a downstream process and has a wide dynamic range suitable for low and high chromatin loadings (1-50µg). The wide dynamic range allows for better results from small sample sizes and provides greater flexibility thus more IP assays can be performed from just one sample
  • The kit is ideal for more difficult primary cell samples and for the enrichment of low abundant transcription factors
  • The Chromatrap® 96 Enzymatic ChIP-seq kit allows the user to perform up to 96 ChIP assays from cell collection through to immunoprecipitation, including up to 10 chromatin sample preparations by enzymatic digestion
  • The kit provides all of the major components required for performing ChIP assays including a DNA purification plate to obtain high quality DNA for NGS library preparation

Positive and negative antibody and primer sets are provided in the kit to give you satisfaction of chromatin preparation.

Products
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Chromatrap HT Enzymatic ChIP-seq Protein A
Product Code : 500216
$1,587.00
Chromatrap HT Enzymatic ChIP-seq Protein A
Product Code : 500216
$1,587.00
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Kit Contents: Chromatrap® 96 HT Protein A plate, Chromatrap® 96 HT balance plate, 96-well collection plate, 96-well balance collection plate, Elution plate, 96-well balance elution plate, Elution and balance plate strip caps, Column conditioning buffer, Wash Buffer 1, Wash Buffer 2, Wash Buffer 3, 1.3M Glycine, Lysis Buffer, ChIP-Seq Elution Buffer, 0.1M NaOH, 5M NaCl, 1M NaHCO3, Hypotonic buffer, Digestion Buffer, Enzymatic Stop Solution, Shearing cocktail, Protease Inhibitor Cocktail (PIC), Proteinase K stop solution and Proteinase K. DNA purification plate, DNA binding buffer, DNA wash buffer and DNA elution buffer. The kit also includes antibody validation controls: H3K4me3 and IgG along with a Forward and Reverse primer set for GAPDH (human promoter region).

Chromatrap HT Enzymatic ChIP-seq Protein G
Product Code : 500217
$1,587.00
Chromatrap HT Enzymatic ChIP-seq Protein G
Product Code : 500217
$1,587.00
Download Protocol
+ Add To Basket

Kit Contents: Chromatrap® 96 HT Protein G plate, Chromatrap® 96 HT balance plate, 96-well collection plate, 96-well balance collection plate, Elution plate, 96-well balance elution plate, Elution and balance plate strip caps, Column conditioning buffer, Wash Buffer 1, Wash Buffer 2, Wash Buffer 3, 1.3M Glycine, Lysis Buffer, ChIP-Seq Elution Buffer, 0.1M NaOH, 5M NaCl, 1M NaHCO3, Hypotonic buffer, Digestion Buffer, Enzymatic Stop Solution, Shearing cocktail, Protease Inhibitor Cocktail (PIC), Proteinase K stop solution and Proteinase K. DNA purification plate, DNA binding buffer, DNA wash buffer and DNA elution buffer. The kit also includes antibody validation controls: H3K4me3 and IgG along with a Forward and Reverse primer set for GAPDH (human promoter region).

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