Chromatrap® ChIP-validated Antibodies can now be supplied with the correct matching primer sets to cover all aspects of ChIP validation.
Many common epigenetic targets of interest are now available as combined antibody & primer sets from Chromatrap®.
Chromatrap®’s MeDIP Validated 5mC Antibody with Positive and Negative Primer Set provides a complete set of tools to assist with a successful MeDIP assay. Including: 5mC antibody, control mouse IgG, positive and negative primer sets. The MeDIP validated 5mC Antibody with Positive and Negative Primer Sets is not suitable for use with non-human species.
Background:
5mC (5-Methylcytosine) is a region of DNA that has been epigenetically modified through the addition of a methyl group at the 5th carbon position of cytosine. DNA methylation is a repressive histone modification that is associated with switching off gene expression. This methylation generally occurs at CpG islands and is also associated with genomic imprinting. Aberrant DNA methylation is linked to disease including many cancers.
A mouse IgG is included in this Antibody Primer Set as a negative control for the MeDIP experiment.
The positive primer set included in this Antibody Primer Set recognises an imprinted gene, a source of hypermethylation. It is therefore an appropriate target for the 5mC antibody. The negative gene target included recognises a hypomethylated, protein-coding gene.
Chromatrap®’s ChIP Validated H3K27me3 Antibody with Positive and Negative Primer Sets provides a complete set of tools to assist with a successful ChIP assay. Including: H3K27me3 antibody, control rabbit IgG, positive and negative primer sets. The ChIP Validated H3K27me3 Antibody with Positive and Negative Primer Sets is not suitable for use with non-human species.
Background:
Histone 3 (H3) is one of the core histone proteins, comprising the protein component of chromatin. H3 is ubiquitous within chromosomes and can be found bound to most gene sequences throughout the genome. H3K27me3 is a histone mark associated with repressive transcription and a closed chromatin structure.
A rabbit IgG is included in this Antibody Primer Set as a negative control for the ChIP experiment.
As H3K27me3 is associated with turning transcription off, the positive primer set included in this Antibody Primer Set recognises a gene associated with repressing transcription. The negative gene target included recognises a gene that is associated with active transcription throughout the genome.
Chromatrap®’s ChIP Validated H3K4me3 Antibody with Positive and Negative Primer Sets provides a complete set of tools to assist with a successful ChIP assay. Including: H3K4me3 antibody, control rabbit IgG, positive and negative primer sets. The ChIP Validated H3K4me3 Antibody with Positive and Negative Primer Sets is not suitable for use with non-human species.
Background:
Histone 3 (H3) is one of the core histone proteins, comprising the protein component of chromatin. H3 is ubiquitous within chromosomes and can be found bound to most gene sequences throughout the genome. Tri-methylation of lysine 4 is associated with transcriptional start sites and gene activation.
A rabbit IgG is included in this Antibody Primer Set as a negative control for the ChIP experiment.
H3K4me3 is associated with activating transcription; the positive primer set included in this Antibody Primer Set recognises a gene associated with active transcription at multiple loci. The negative gene target included recognises a gene that is associated with repressive transcription.
Chromatrap®’s ChIP Validated RNA Pol II (S) Antibody with Positve and Negative Primer Sets provides a complete set of tools to assist with a successful ChIP assay. Including: RNA Pol II (S) antibody, control mouse IgG, positive and negative primer sets. The ChIP Validated RNA Pol II (S) Antibody with Positive and Negative Primer Sets is not suitable for use with non-human species.
Background:
RNA polymerase II catalyses the transcription of DNA into mRNA and other small nuclear RNAs in eukaryotes. It contains a CTD consisting of conserved heptapeptide repeats. Phosphorylation occurs at serine and therine residues located in the CTD repeats to activate the RNA pol II. RNA pol II binds to several transcription factors in order to initiate transcription and therefore serves as an abundant antibody target for ChIP.
A mouse IgG is included in this Antibody Primer Set as a negative control for the ChIP experiment.
The positive primer set included in this Antibody Primer Set recognises a gene associated with active transcription due to the role of RNA Pol II (S) in catalysing transcription. The negative gene target included recognises a gene that is associated with repressive transcription.
Chromatrap®’s ChIP Validated H3K9me3 Antibody with Positive and Negative Primer Sets provides a complete set of tools to assist with a successful ChIP assay. Including: H3K9me3 antibody, control mouse IgG, positive and negative primer sets. The ChIP Validated H3K9me3 Antibody with Positive and Negative Primer Sets is not suitable for use with non-human species.
Background:
Histone 3 tri methyl K9 (H3K9me3) is a histone mark associated with repressed chromatin. It condenses and compacts the chromatin, restricting the transcription machinery from binding and carrying out gene expression. It is a marker of constitutive heterochromatin, and generally associated with gene silencing. As a histone mark, it is widely found throughout human cell lines, serving as an abundant antibody target for ChIP.
A mouse IgG is included in this Antibody Primer Set as a negative control for the ChIP experiment.
H3K9me3 is usually associated with closed chromatin and repressive transcription. The positive primer set included in this Antibody Primer Set recognises a protein-coding gene that is present when transcription is switched off. The negative gene target included recognises a gene that is associated with open chromatin and active transcription at multiple loci in the genome.
Chromatrap®’s ChIP Validated AR Antibody with Positve and Negative Primer Sets provides a complete set of tools to assist with a successful ChIP assay. Including: AR antibody, control sheep IgG, positive and negative primer sets. The ChIP Validated AR Antibody with Positive and Negative Primer Sets is not suitable for use with non-human species.
Background:
The androgen receptor (AR) is a steroid hormone receptor involved in the signalling of testosterone. It is a regulatory gene that controls gene expression through binding to androgen receptor elements (AREs). The AR is associated with both normal prostate and cancerous prostate cell growth at abnormal levels of the gene (Lamb et al, 2014, BJUI). Androgens are also heavily involved in the pathogenesis of endometrial cancer (D.A. Gibson et al, 2014, Endocr Relat Cancer). DHT (dihydrotestosterone) is a natural androgen hormone that can activate the androgen receptor (Mostaghel, 2014, IJBS).
A sheep IgG is included in this Antibody Primer Set as a negative control for the ChIP experiment.
The androgen receptor positive primer set recognises a protein-coding gene that is regulated by androgen. The negative gene target included recognises a gene that is not present at the androgen receptor.
Chromatrap®’s ChIP Validated Sin3a Antibody with Positive and Negative Primer Sets provides a complete set of tools to assist with a successful ChIP assay. Including: Sin3a antibody, control sheep IgG, positive and negative primer sets. The ChIP Validated Sin3a Antibody with Positive and Negative Primer Sets is not suitable for use with non-human species.
Background:
Sin3a (Switch independent 3 homologue) is a transcriptional repressor and protein-encoding gene. It interacts with co-repressors and DNA-binding proteins such as REST, MXl1 and MXD1-MAX that allow Sin3a to bind to DNA and repress transcriptional activity. It acts interdependently with OGT (O-linked N-acetylglucosamine) to repress transcription alongside with histone deacetylation.
A sheep IgG is included in this Antibody Primer Set as a negative control for the ChIP experiment.
The Sin3a positive primer set recognises the hypermethylated promoter of a gene that binds to Sin3a, resulting in the repression of transcription. The negative gene target included recognises a gene that is associated with active transcription.
Chromatrap®’s ChIP Validated H3K14ac Antibody with Positive and Negative Primer Sets provides a complete set of tools to assist with a successful ChIP assay. Including: H3K14ac antibody, control sheep IgG, positive and negative primer sets. The ChIP Validated H3K14ac Antibody with Positive and Negative Primer Sets is not suitable for use with non-human species.
Background:
Histone 3 (H3) is one of the core histone proteins, comprising the protein component of chromatin. H3 is ubiquitous within chromosomes and can be found bound to most gene sequences throughout the genome. Acetylation of histone H3 occurs at several different lysine positions on the histone tail and is catalysed by a group of enzymes called histone acetyltransferases (HATs). The histone acetylation mark H3K14ac can indicate enhancer sites; helping to identify active enhancers from poised ones. H3K14ac is associated with active transcription and can be found at the start site of actively transcribed genes.
A rabbit IgG is included in this Antibody Primer Set as a negative control for the ChIP experiment.
The H3K14ac positive primer set recognises the promoter of a gene associated with active transcription and is a suitable target for this antibody. The negative gene target included recognises a protein-coding gene that is present when transcription is switched off.
Chromatrap®’s ChIP Validated H3K27ac Antibody with Positive and Negative Primer Sets provides a complete set of tools to assist with a successful ChIP assay. Including: H3K27ac antibody, control sheep IgG, positive and negative primer sets. The ChIP Validated H3K27ac Antibody with Positive and Negative Primer Sets is not suitable for use with non-human species.
Background:
Histone 3 (H3) is one of the core histone proteins, comprising the protein component of chromatin. H3 is ubiquitous within chromosomes and can be found bound to most gene sequences throughout the genome. Acetylation of histone H3 occurs at several different lysine positions on the histone tail and is catalysed by a group of enzymes called histone acetyltransferases (HATs). The acetylation of lysine 27 on histone 3 can be used to identify active enhancer sites, separating them from poised enhancers. Histone acetylation is associated with active transcription through its roles in chromatin remodelling and gene activation.
A rabbit IgG is included in this Antibody Primer Set as a negative control for the ChIP experiment.
The H3K27ac positive primer set recognises the promoter of a gene associated with active transcription and is a suitable target for this antibody. The negative gene target included recognises a gene associated with repressive transcription.
Chromatrap®’s ChIP Validated H3K4me1 Antibody with Positive and Negative Primer Sets provides a complete set of tools to assist with a successful ChIP assay. Including: H3K4me1 antibody, control rabbit IgG, positive and negative primer sets. The ChIP Validated H3K4me1 Antibody with Positive and Negative Primer Sets is not suitable for use with non-human species.
Background:
Histone 3 (H3) is one of the core histone proteins, comprising the protein component of chromatin. H3 is ubiquitous within chromosomes and can be found bound to most gene sequences throughout the genome. Monomethlyation of lysine 4 on histone 4 (H3K4me1) is a histone modification associated with open chromatin and active transcription. The presence of H3K4me1 generally indicates an enhancer region of DNA.
A rabbit IgG is included in this Antibody Primer Set as a negative control for the ChIP experiment.
The H3K4me1 positive primer set recognises the promoter of the GAPDH gene, associated with active transcription and is a suitable target for this antibody. The negative primer set recognises the transcription start site of GAPDH.
Chromatrap®’s ChIP Validated H4K5ac Antibody with Positive and Negative Primer Sets provides a complete set of tools to assist with a successful ChIP assay. Including: H4K5ac antibody, control rabbit IgG, positive and primer sets. The ChIP Validated H4K5ac Antibody with Positive and Negative Primer Sets is not suitable for use with non-human species.
Background:
Histone 4 (H4) is one of the five core histone proteins, comprising the protein component of chromatin. H4 is ubiquitous within chromosomes and can be found bound to most gene sequences throughout the genome. Acetylation of lysine 5 on histone 4 (H4K5ac) is associated with open chromatin and active gene transcription. H4K5ac has been shown to have roles in epigeneticbookmarking, a process where genetic information is passed onto daughter cells during cell division.
A rabbit IgG is included in this Antibody Primer Set as a negative control for the ChIP experiment.
The H4K5ac positive primer set recognises the promoter of the GAPDH gene, associated with active transcription and is a suitable target for this antibody. The negative primer set recognises the MLH1 gene.
Chromatrap®’s ChIP Validated H4K12ac Antibody with Positive and Negative Primer Set provides a complete set of tools to assist with a successful ChIP assay. Including: H4K12ac antibody, control rabbit IgG, positive and negative primer sets. The ChIP Validated H4K12ac Antibody with Positive and Negative Primer Sets is not suitable for use with non-human species.
Background:
Histone 4 (H4) is one of the core histone proteins, comprising the protein component of chromatin. H4 is ubiquitous within chromosomes and can be found bound to most gene sequences throughout the genome. Lysine 12 on histone 4 (H4K12) can only be acetylated and is not associated with methylation. The histone modification H4K12ac is associated with active promoter regions and has roles in activating the transcription of genes, in particular genes with roles in memory and learning. H4K12ac can have an influence on paternal inheritance in the zygote, indicating the importance of this mark for embryo development.
A rabbit IgG is included in this Antibody Primer Set as a negative control for the ChIP experiment.
The H4K12ac positive primer set recognises the GREB1 gene. The negative primer set recognises the SAT2 gene.
Chromatrap®’s ChIP Validated H3K9me1 Antibody with Positive and Negative Primer Sets provides a complete set of tools to assist with a successful ChIP assay. Including: H3K9me1 antibody, control rabbit IgG, positive and negative primer sets. The ChIP Validated H3K9me1 Antibody with Positive and Negative Primer Sets is not suitable for use with non-human species.
Background:
Histone 3 is one of the core histone proteins, comprising the protein component of chromatin. Histones 3 monomethyl lysine 9 (H3K9me1) is a histone mark associated with gene activation. It is enriched at the transcriptional start site of genes and is detected in active gene promoters, where it can characterise active gene transcription.
A rabbit IgG is included in this Antibody Primer Set as a negative control for the ChIP experiment.
The H3K9me1 positive primer set recognises the promoter of the SAT2 gene. The negative gene target included recognises the promoter of the GAPDH gene.
Chromatrap®’s ChIP Validated H3K9me2 Antibody with Positive and Negative Primer Sets provides a complete set of tools to assist with a successful ChIP assay. Including: H3K9me2 antibody, control rabbit IgG, positive and negative primer sets. The ChIP Validated H3K9me2 Antibody with Positive and Negative Primer Sets is not suitable for use with non-human species.
Background:
Histone 3 is one of the core histone proteins, comprising the protein component of chromatin. Histone 3 tri methyl K9 (H3K9me2) is a histone mark generally associated with repressed chromatin. It condenses and compacts the chromatin, restricting the transcription machinery from binding and carrying out gene expression. It is often found at silenced genes and is a mark of transcriptional repression.
A rabbit IgG is included in this Antibody Primer Set as a negative control for the ChIP experiment.
The H3K9me2 positive primer set recognises the promoter of the SAT2 gene. The negative gene target included recognises the c-fos gene.
Applications: ChIP
Source: Rabbit
Concentration: 1mg/ml
Type: Monoclonal
Size: 50μl
Purification: Protein A (affinity purified)
Specificity: Human
Storage Conditions: The H3K9me2 antibody should be stored at +4°C. Rabbit IgG and primer sets should all be stored at -20°C (Avoid multiple freeze/thaw cycles as this may denature the antibody and degrade the primer sets)
