The use of high quality and specific ChIP validated antibodies is essential for the success of a ChIP assay. The antibody must recognise and bind to native protein that is bound to DNA. Antibodies from other applications do not always work well in ChIP.
Histone 3 (H3) one of the core histone proteins, comprising the protein component of chromatin. H3 is ubiquitous within chromosomes and can be found bound to most gene sequences throughout the genome. It therefore serves as an abundant antibody target for ChIP and recognises the C-terminal peptide of human histone H3.
RNA polymerase II catalyzes the transcription of DNA into mRNA and other small nuclear RNAs in eukaryotes. It contains a CTD consisting of conserved heptapeptide repeats. Phosphorylation occurs at serine and therine residues located in the CTD repeats to activate the RNA pol II.
RNA pol II binds to several transcription factors in order to initiate transcription and therefore serves as an abundant antibody target for ChIP. This antibody recognises the C-terminal repeat of the largest subunit of RNA polymerase II. It recognises both unphosphorylated and phosphorylated form.
Histone 3 tri methyl K9 (H3K9me3) is a histone mark associated with repressed chromatin. It condenses and compacts the chromatin, restricting the transcription machinery from binding and carrying out gene expression. It is a marker of constitutive heterochromatin, and generally associated with gene silencing.
As a histone mark, it is widely found throughout human cell lines, serving as an abundant antibody target for ChIP. This antibody recognises the synthetic peptide of Human Histone H3, tri methylated at K9.
5mC (5-Methylcytosine) is a region of DNA that has been epigenetically modified through the addition of a methyl group at the 5th carbon position of cytosine. DNA methylation is a repressive histone modification that is associated with switching off gene expression. This methylation generally occurs at CpG islands and is also associated with genomic imprinting. Aberrant DNA methylation is linked to disease including many cancers.
Histone 3 (H3) is one of the core histone proteins, comprising the protein component of chromatin. H3 is ubiquitous within chromosomes and can be found bound to most gene sequences throughout the genome. Tri-methylation of lysine 4 is associated with transcriptional start sites and gene activation.
This antibody recognizes a peptide corresponding to trimethyl-histone H3 (Lys 4).
Histone 3 (H3) is one of the core histone proteins, comprising the protein component of chromatin. H3 is ubiquitous within chromosomes and can be found bound to most gene sequences throughout the genome. Acetylation of histone H3 occurs at several different lysine positions on the histone tail and is catalysed by a group of enzymes called histone acetyltransferases (HATs). The acetylation of lysine 27 on histone 3 can be used to identify active enhancer sites, separating them from poised enhancers. Histone acetylation is associated with active transcription through its roles in chromatin remodelling and gene activation.
This antibody recognises an acetyl-peptide corresponding to the Acetyl-Histone H3 (Lys 27).
Histone 3 (H3) is one of the core histone proteins, comprising the protein component of chromatin. H3 is ubiquitous within chromosomes and can be found bound to most gene sequences throughout the genome. Acetylation of histone H3 occurs at several different lysine positions on the histone tail and is catalysed by a group of enzymes called histone acetyltransferases (HATs). The histone acetylation mark H3K14ac can indicate enhancer sites; helping to identify active enhancers from poised ones. H3K14ac is associated with active transcription and can be found at the start site of actively transcribed genes.
This antibody recognises an acetyl-peptide corresponding to the Acetyl-Histone H3 (Lys 14).
Histone 3 (H3) is one of the core histone proteins, comprising the protein component of chromatin. H3 is ubiquitous within chromosomes and can be found bound to most gene sequences throughout the genome. H3K27me3 is a histone mark associated with repressive transcription and a closed chromatin structure.
This antibody recognises a trimethyl-peptide corresponding to trimethyl-Histone H3 (Lys 27).
Immunoglobulin G (IgG) is an antibody isotype. It is comprised of two antigen binding sites and is created and released by plasma B cells. Purified rabbit IgG is used as a negative experimental control in many applications including Chromatin Immunoprecipitation (ChIP).
Immunogen is Rabbit IgG, polyclonal, isotype control.
Histone 3 (H3) is one of the core histone proteins, comprising the protein component of chromatin. H3 is ubiquitous within chromosomes and can be found bound to most gene sequences throughout the genome. Monomethlyation of lysine 4 on histone 4 (H3K4me1) is a histone modification associated with open chromatin and active transcription. The presence of H3K4me1 generally indicates an enhancer region of DNA.
Histone 4 (H4) is one of the core histone proteins, comprising the protein component of chromatin. H4 is ubiquitous within chromosomes and can be found bound to most gene sequences throughout the genome. Lysine 12 on histone 4 (H4K12) can only be acetylated and is not associated with methylation. The histone modification H4K12ac is associated with active promoter regions and has roles in activating the transcription of genes, in particular genes with roles in memory and learning. H4K12ac can have an influence on paternal inheritance in the zygote, indicating the importance of this mark for embryo development.
Histone 3 is one of the core histone proteins, comprising the protein component of chromatin. Histones 3 monomethyl lysine 9 (H3K9me1) is a histone mark associated with gene activation. It is enriched at the transcriptional start site of genes and is detected in active gene promoters, where it can characterise active gene transcription.
Histone 3 is one of the core histone proteins, comprising the protein component of chromatin. Histone 3 tri methyl K9 (H3K9me2) is a histone mark generally associated with repressed chromatin. It condenses and compacts the chromatin, restricting the transcription machinery from binding and carrying out gene expression. It is often found at silenced genes and is a mark of transcriptional repression.
Histone H3K9ac is a histone mark associated with active promoters. Acetylation of H3 at lysine 9 near transcription start sites is related to activation of transcription.
