Why using Chromatrap® kits is the method of choice
At Chromatrap, we are constantly working to ensure we uphold the standard of our customer service – and a big part of the process is receiving feedback from our customers. We recently got in touch with Fengtian Wang, who visited us at our booths at Experimental Biology 2017 and the Annual American Association for Cancer Research Meeting, about his experience with the Chromatrap® ChIP-seq kit.
What do you do at LSU Health Sciences Centre?
I am currently a third year graduate student at department of Biochemistry at Louisiana State University Health Sciences Centre in Shreveport, LA.
Can you describe a typical working day?
As a graduate student, the majority of my time is devoted to bench work - my lab is a basic research lab focusing on prostate cancer. My project is focused on studying the role of a transcription factor named PDEF in prostate cancer progression in cell lines as well as in mouse model.
During a typical working day, I will review the latest published literatures related to my research and then start my bench work including cell culture, western blot and sometimes chromatin immunoprecipitation (ChIP).
When did you first hear about Chromatrap®?
I first heard about Chromatrap in 2016 at the Annual Meeting of American Association of Cancer Research (AACR) in New Orleans. I was struggling with my own chromatin immunoprecipitation experiment due to the low concentration of the final DNA product.
My lab was using old school magnetic beads for ChIP and the amount of DNA was too low to prepare the library for sequencing. I came across Chromatrap at the exhibition zone and learnt that they have a column based CHIP-seq kit, and would be willing to offer a free trial with 6 columns.
Why do you like using Chromatrap®?
It is easy to start and time saving. My lab has no previous experience with CHIP-seq, and the traditional method is hard to get the hang of and it consumes a lot of time and money, so it was a welcome change.
Are you intending to publish the ChIP data you have received when using Chromatrap?
Yes. We are currently writing up a manuscript using the ChIP-qPCR data we got using Chromatrap. We also got very promising ChIP-seq data which we are currently analysing.
What makes Chromatrap® unique?
I think it is the cost-effective method of Chromatrap. Magnetic beads are costly and can yield inconsistent results, while the technology Chromatrap yields consistent results and is less expensive. Chromatrap also has a very good customer service.
Why would you recommend Chromatrap® to other researchers?
It is a very good method for researchers who have had few experiences with ChIP-seq or ChIP-qPCR. The kit contains all the materials including positive and negative control antibody. It also gives reproducible results.
We always love hearing how our solid state platform for chromatin immunoprecipitation has helped the research of the many laboratories and research centres around the world, and would love to hear how Chromatrap has helped your research.