typical ChIP process
08 Jul 2016
How Chromatrap® can complete a typical ChIP process in under 5 hours

Chromatrap® is a more efficient, sensitive and robust method of chromatin immunoprecipitation meaning that it can save valuable time in the laboratory. Chromatrap®’s technology is also available in a variety of formats, including ChIP qPCR with spin columns, ChIP-seq with spin columns, Native ChIP, Enzymatic ChIP kits for qPCR and many more!

These kits can be used in the lab and in comparison with traditional methods Chromatrap® can halve the time spent working on the ChIP process. Traditional methods can take on average 10 hours but with Chromatrap® it can be under 5 hours.

Here is an example of how Chromatrap® is a simpler and faster process with less manual handling:

chromatrap timeline

Antibody/Protein binding to chromatin

  • Traditional Method: 2-4 hours
  • Chromatrap® for qPCR: 1 hour

Wash steps to remove non-specific binding

  • Traditional Method: 5-15 minutes

The wash steps in the traditional method requires manual pipette handling and incubation which can take a lot more time.

  • Chromatrap® for qPCR: 30 seconds

With Chromatrap® there is a simple wash-through steps and no incubation is needed.

Elute to release chromatin

  • Traditional Method

At this stage the process requires resuspension and bead handling by manual pipette handling.

  • Chromatrap® for qPCR

Only a simple centrifugation is needed

DNA purification

  • Traditional Method

There is further manual handling as the columns need to be separated which will result in inevitable loss of DNA.

  • Chromatrap® for qPCR

DNA purification is not required with Chromatrap®.

The above details how Chromatrap® is able to reduce the time spent in the laboratory by minimising the need for manual handling. By reducing the time spent using a pipette and with no incubation required, it means that results are achieved faster.

We also have more information about Chromatrap® and how it can be used in a special presentation on SlideShare.

 

You can reduce your laboratory time by using our Chromatrap® ChIP kits - to find out which one is best suited for your research, consult our step-by-step guide.

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