What is Chromatrap?

Chromatrap® improved ChIP assay performance

Chromatrap® uses a solid phase porous polymer functionalised with either protein A or protein G allowing the chromatin capture to be more efficient than bead based methods.

  • Levels of DNA pull down up to 25 times higher than with bead based procedures.
  • Excellent DNA enrichment with signal to noise ratios typically 2 to 3 times better than competing procedures.
  • High performance with small sample sizes; as few as 7500 cells per assay.

Chromatrap®: a faster, easier technique

The Chromatrap® approach is preferable to other methods because:

  • The use of spin columns negates the use of microbeads
  • BioVyon™ protein A discs have a proprietary internal structure, composed of pores that have inner surfaces specifically designed to maximise chromatin capture efficiency
  • The disc base material is chemically inert, reducing incidences of non-specific binding
  • All chemical reactions take place inside the disc, therefore there is no need for suspension or re-suspension by pipette
  • There is no pre-blocking step required
  • The protocol requires washing steps that are fast, efficient and less prone to error
  • The ‘flow through’ characteristics of the column ensure excellent sample mixing and washing during the assay.

Literature Library

News

What is Chromatrap?