Chromatin immunoprecipitation (ChIP) is a technique used to study the association of specific proteins, or their modified isoforms, with defined genomic regions.
In a ChIP assay, DNA-protein complexes (Chromatin) are identified using matching antibodies and the resulting fractions treated to separate the DNA and protein components. Polymerase Chain Reaction (PCR), Real Time PCR, hybridization on microarrays, or direct sequencing are typically used to identify DNA fragments of defined sequence.
Traditional ChIP methods
The traditional ChIP methods, based on sepharose or magnetic beads, involve many steps of separation, pipetting and re-suspension. These sequences can either be done by magnetism or centrifusion, which are repetitive and prone to errors.
Our new Chromatrap® technology
Chromatrap® is a new technology that offers a quicker, easier and more efficient way of undertaking chromatin immunoprecipitation assays. These kits use revolutionary Chromatrap® Pro-A spin columns which contain discs of an inert, porous polymer to which protein A has been covalently attached. During an assay the chromatin/antibody complex is retained by the disc. Flushing with three buffers and an elution step are all that is required to obtain the DNA fragments of interest.